Practical experience versus laboratory test methods

Lab test methods try to imitate reality, often with only mediocre success, but enormous expenditure. Certain test methods illustrate certain effective principles of the product. The experience made during wearing is the best test method by far to experience the mode of action of SILVERPLUS®.

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In a sensory test the renowned independent Hohenstein test institutes have found a distinctive difference between an unfinished used running shirt and a used running shirt that had been finished with SILVERPLUS®.

1. Necessary equipment:
   2 small glass bottles with lid, pipettes, non-pasteurised milk, textile (untreated/treated)
2. Glass bottle 1:
   with untreated textile
3. Glass bottle 2:
   with textile treated with SILVERPLUS®
4. Apply 3 - 4 milk drops with a pipette to the textile
5. Close glass bottles and store warm
   for 2 - 3 days
6. Open glass bottles

The “Dynamic Shake Flask“ test method according to the test standard ASTM-E2149-01/10 is the best test method for demonstrating the antimicrobial effect of SILVERPLUS®. This test method imitates the wearing of textiles.

Dynamic Shake Flask Test
(ASTM-E2149-01/10)
Test principle: The contact between antimicrobially finished textiles and bacteria in a bacterial suspension is ensured by thorough mechanical shaking. The antimicrobial active ingredient does hence not necessarily migrate out of the substrate.

The full antimicrobial effect of SILVERPLUS® by means of free, antimicrobial silver ions is normally attained after a dwell time of 24 hours.

SILVERPLUS® is a non-migrating, antimicrobial technology, which means that it is only effective on the textile and does not form inhibition zones according to the Agar Diffusion Plate Test (SN 195 920).

Test principle: The antimicrobial active ingredient moves out of the substrate (ideal case for migrating products) and forms an unpopulated zone (inhibition zone) in the environment. Only the migrating product shows an inhibition zone.

For proving the effect of SILVERPLUS® the Agar Diffusion Plate Test is unsuitable.

• 1 = Migrating product (inhibition zone)
• 2 = SILVERPLUS® (non-migrating, no inhibition zone)
• 3 = Conventional product (non-migrating, no inhibition zone)
• U = Untreated textile (grown with bacteria)